By Simon P. Langdon
Specialist researchers describe intimately their most efficient and up to date equipment for starting to be melanoma cells within the laboratory. there are ways to represent and authenticate phone traces, to isolate and advance particular kinds of melanoma cells, and to strengthen new phone line types. practical assays are supplied to guage clonogenicity, cellphone proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and mobilephone cycle parameters. different equipment enable the amendment of melanoma cells for transfection, improvement of drug resistance, immortalization, and move in vivo; the co-culture of other phone kinds; and the detection and therapy of infection.
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19. Masters, J. , Thomson, J. , Daly-Burns, B. , (2001) Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. USA 98, 8012–8017. 20. Rowley, J. D. (1973) A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature 243, 290–293. 21. Seabright, M. (1973) Improvement of trypsin method for banding chromosomes. Lancet1, 1249–1250. 22. , and Johansson, C. (1970) Differential binding of alkylating fluorochromes in human chromosomes.
Even with the advent of DNA profiling, which is technically more feasible for many laboratories, cytogenetic analysis retains a useful complementary approach to defining and characterizing an individual cancer cell line or culture. It possesses some important advantages over profiling. , the role of the Philadelphia chromosome in chronic myeloid leukemia (20). Microscopic observation allows the monitoring of diverging subgroups that may not be detected by biochemical sampling of whole populations.
50 Dirks and Drexler Fig. 1. AmpFLP VNTR DNA profiles of prominent human cross-contaminating cell lines. Genomic DNA of cell lines was used to amplify alleles from Apo-B1, D17S5, D1S80, and D2S44. After size determination of the amplicons from each loci on a separate gel using gel analyzing software, the data are entered in a database, creating a specific DNA profile for each cell line. Lanes 1–13: 1, HeLa, human cervix carcinoma; 2, CCRF-CEM, human T-cell leukemia; 3, CTV-1, human acute myeloid leukemia; 4, NALM-6, human B cell precursor leukemia; 5, HEL, human erythroleukemia; 6, SCLC-21H, human small cell lung carcinoma; 7, DU-145, human prostate carcinoma; 8, T-24, human urinary bladder carcinoma; 9, COLO-800, human melanoma; 10, U-937, human histiocytic lymphoma; 11, HT-29, human colon adenocarcinoma; 12, SK-HEP-1, human liver adenocarcinoma; 13, K-562, human chronic myeloid leukemia in blast crisis.
Cancer Cell Culture. Methods and Protocols by Simon P. Langdon