Cancer Cell Culture. Methods and Protocols - download pdf or read online

By Simon P. Langdon

ISBN-10: 1588290794

ISBN-13: 9781588290793

Specialist researchers describe intimately their most efficient and up to date equipment for starting to be melanoma cells within the laboratory. there are ways to represent and authenticate phone traces, to isolate and advance particular kinds of melanoma cells, and to strengthen new phone line types. practical assays are supplied to guage clonogenicity, cellphone proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and mobilephone cycle parameters. different equipment enable the amendment of melanoma cells for transfection, improvement of drug resistance, immortalization, and move in vivo; the co-culture of other phone kinds; and the detection and therapy of infection.

Show description

Read Online or Download Cancer Cell Culture. Methods and Protocols PDF

Best oncology books

Download e-book for iPad: Signalisation cellulaire et cancer by Jacques Robert

Plus que jamais, les oncologues ont besoin de l’apport de los angeles biologie, d’abord pour comprendre les cancers, mais surtout pour les traiter. Plus de six hundred essais th? rapeutiques sont en cours en 2010 pour ? valuer les th? rapies cibl? es. Afin de s’y retrouver dans le d? dale des voies de signalisation, dans l. a. great quantity des r?

Herbert B. Newton, Mark G. Malkin's Neurological complications of systemic cancer and PDF

Neurological issues of Systematic melanoma and Antineoplastic treatment presents an in-depth overview of universal manifestations concerning neurology that ensue in sufferers with systemic melanoma. those comprise mind metastases, spinal twine compression, cerebrovascular occasions, and leptomeningeal illness.

Phase I Cancer Clinical Trials: A Practical Guide by Elizabeth A. Eisenhauer, Christopher Twelves, Marc Buyse PDF

Part I trials are a serious first step within the research of novel melanoma healing methods. Their basic ambitions are to spot the advised dose, agenda and pharmacologic habit of recent brokers or new mixtures of brokers and to explain the adversarial results of therapy. In melanoma therapeutics, such reports have specific demanding situations.

Targeted therapies in oncology by Giuseppe Giaccone, Jean-Charles Soria PDF

Because the final variation of this e-book, significant advances were made in our knowing of key pathways that keep an eye on tumor development. This has ended in the advance of latest anticancer brokers that experience the facility to dam the task of proteins serious about neoplastic telephone improvement and proliferation.

Extra info for Cancer Cell Culture. Methods and Protocols

Sample text

19. Masters, J. , Thomson, J. , Daly-Burns, B. , (2001) Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. USA 98, 8012–8017. 20. Rowley, J. D. (1973) A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature 243, 290–293. 21. Seabright, M. (1973) Improvement of trypsin method for banding chromosomes. Lancet1, 1249–1250. 22. , and Johansson, C. (1970) Differential binding of alkylating fluorochromes in human chromosomes.

Even with the advent of DNA profiling, which is technically more feasible for many laboratories, cytogenetic analysis retains a useful complementary approach to defining and characterizing an individual cancer cell line or culture. It possesses some important advantages over profiling. , the role of the Philadelphia chromosome in chronic myeloid leukemia (20). Microscopic observation allows the monitoring of diverging subgroups that may not be detected by biochemical sampling of whole populations.

50 Dirks and Drexler Fig. 1. AmpFLP VNTR DNA profiles of prominent human cross-contaminating cell lines. Genomic DNA of cell lines was used to amplify alleles from Apo-B1, D17S5, D1S80, and D2S44. After size determination of the amplicons from each loci on a separate gel using gel analyzing software, the data are entered in a database, creating a specific DNA profile for each cell line. Lanes 1–13: 1, HeLa, human cervix carcinoma; 2, CCRF-CEM, human T-cell leukemia; 3, CTV-1, human acute myeloid leukemia; 4, NALM-6, human B cell precursor leukemia; 5, HEL, human erythroleukemia; 6, SCLC-21H, human small cell lung carcinoma; 7, DU-145, human prostate carcinoma; 8, T-24, human urinary bladder carcinoma; 9, COLO-800, human melanoma; 10, U-937, human histiocytic lymphoma; 11, HT-29, human colon adenocarcinoma; 12, SK-HEP-1, human liver adenocarcinoma; 13, K-562, human chronic myeloid leukemia in blast crisis.

Download PDF sample

Cancer Cell Culture. Methods and Protocols by Simon P. Langdon


by Edward
4.2

Rated 4.95 of 5 – based on 19 votes

Categories: Oncology