By John H. Rothman, Andrew Singson
An up-to-date version of the classic Methods in telephone Biology quantity forty eight, this book emphasizes assorted equipment and applied sciences had to examine C. elegans, either as an built-in organism and as a version procedure for examine inquiries in telephone, developmental, and molecular biology, in addition to in genetics and pharmacology. by way of directing its audience to tried-and-true and state of the art recipes for research, this accomplished assortment is meant to steer investigators of C. elegans for years yet to come. *Diverse, updated concepts covered will be priceless to the broadening neighborhood of C. elegans researchers for future years* Chapters written by means of leaders within the box* attempted and actual tools bring busy researchers a one-stop compendium of crucial protocols
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Additional resources for Caenorhabditis elegans: Molecular Genetics and Development
A. (2006). Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans. Biotechniques 41, 583–588. Sigurdson, D. , Herman, R. , Horton, C. , Kari, C. , and Pratt, S. E. (1986). An X-autosome fusion chromosome of Caenorhabditis elegans. Mol. Gen. Genet. 202, 212–218. Sigurdson, D. , Spanier, G. , and Herman, R. K. (1984). Caenorhabditis elegans deficiency mapping. Genetics 108, 331–345. Sun, A. , and Lambie, E. J. (1997). gon-2, a gene required for gonadogenesis in Caenorhabditis elegans.
As for alleles, the name is preceded by the small letter italic designation of the originating laboratory. If the SC carries a mutation in a known gene, the gene is described using square brackets following the SC name, for example, the reciprocal translocation eT1, which has a breakpoint in unc-36 (III), is eT1[unc-36] (III;V). The components of a reciprocal translocation can be identified by their pairing properties. Chromosome identity in C. , 1988b). Thus, eT1 (III) pairs with the normal chromosome III, and eT1 (V) with the normal chromosome V.
Jones, Zoe Lohn and Ann M. Rose Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada Abstract I. Introduction A. Nomenclature for Chromosomal Rearrangements II. Chromosomal Rearrangements A. Translocations B. Inversions C. Deletions (Deficiencies) D. Duplications III. Characterization of Chromosomal Rearrangements A. Restriction Enzyme Digestion of Single Nucleotide Polymorphisms (snip-SNP) B. Oligonucleotide Array Comparative Genome Hybridization (aCGH) C.
Caenorhabditis elegans: Molecular Genetics and Development by John H. Rothman, Andrew Singson
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