By Gérald Thouand, Robert Marks
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Additional resources for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3
Based on the above application of micro-electrochemical biochips in bacterial bioluminescent assays, the optimization of analytical performances has been reported . Speciﬁcally, the authors developed two independent protocols for the modiﬁcation of a working electrode integrated in an electrochemical biochip. Thus, one protocol used Cu–Au micropillars to increase the electrode’s effective area, while the second one used conductive polypyrrole (PPy) ﬁlms. It was noticed that, Fig. 4 A silicon-based micro-chip with four differentially sized electrochemical micro-chambers (a).
Speciﬁcally, the ﬁrst strategy was to modify the length of the DNA segment containing the promoter region. The second approach introduced a random gene mutation via a directed evolution process, whereas the third approach introduced more speciﬁc site mutations in the promoter sequence (−35 and −10 regions). The ﬁnal strategy was the duplication of the promoter sequence to increase the binding sites for RNA polymerase. As a consequence, the bioluminescent sensitivity, kinetic response (earlier response time), and intensity of emitted light were signiﬁcantly improved.
Anal Bioanal Chem 400(4):1051–1060 68. Ben-Yoav H, Amzel T, Sternheim M, Belkin S, Rubin A, Shacham-Diamand Y, Freeman A (2011) Signal amelioration of electrophoretically deposited whole-cell biosensors using external electric ﬁelds. Electrochim Acta 56(26):9666–9672 69. Ben-Yoav H, Freeman A, Sternheim M, Shacham-Diamand Y (2011) An electrochemical impedance model for integrated bacterial bioﬁlms. Electrochim Acta 56(23):7780–7786 70. Besra L, Liu M (2007) A review on fundamentals and applications of electrophoretic deposition (EPD).
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand, Robert Marks
Categories: Basic Science