Download e-book for iPad: Bioluminescence: Fundamentals and Applications in by Gérald Thouand, Robert Marks

By Gérald Thouand, Robert Marks

ISBN-10: 3319274058

ISBN-13: 9783319274058

ISBN-10: 3319274074

ISBN-13: 9783319274072

This publication evaluation sequence provides present tendencies in sleek biotechnology. the purpose is to hide all elements of this interdisciplinary expertise the place wisdom, equipment and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and desktop technology. Volumes are geared up topically and supply a accomplished dialogue of advancements within the respective box over the last 3-5 years. The sequence additionally discusses new discoveries and purposes. distinct volumes are devoted to chosen subject matters which concentrate on new biotechnological items and new techniques for his or her synthesis and purification. mostly, distinct volumes are edited via famous visitor editors. The sequence editor and writer will despite the fact that constantly be happy to obtain feedback and supplementary info. Manuscripts are authorised in English.

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Additional resources for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3

Sample text

Based on the above application of micro-electrochemical biochips in bacterial bioluminescent assays, the optimization of analytical performances has been reported [47]. Specifically, the authors developed two independent protocols for the modification of a working electrode integrated in an electrochemical biochip. Thus, one protocol used Cu–Au micropillars to increase the electrode’s effective area, while the second one used conductive polypyrrole (PPy) films. It was noticed that, Fig. 4 A silicon-based micro-chip with four differentially sized electrochemical micro-chambers (a).

Specifically, the first strategy was to modify the length of the DNA segment containing the promoter region. The second approach introduced a random gene mutation via a directed evolution process, whereas the third approach introduced more specific site mutations in the promoter sequence (−35 and −10 regions). The final strategy was the duplication of the promoter sequence to increase the binding sites for RNA polymerase. As a consequence, the bioluminescent sensitivity, kinetic response (earlier response time), and intensity of emitted light were significantly improved.

Anal Bioanal Chem 400(4):1051–1060 68. Ben-Yoav H, Amzel T, Sternheim M, Belkin S, Rubin A, Shacham-Diamand Y, Freeman A (2011) Signal amelioration of electrophoretically deposited whole-cell biosensors using external electric fields. Electrochim Acta 56(26):9666–9672 69. Ben-Yoav H, Freeman A, Sternheim M, Shacham-Diamand Y (2011) An electrochemical impedance model for integrated bacterial biofilms. Electrochim Acta 56(23):7780–7786 70. Besra L, Liu M (2007) A review on fundamentals and applications of electrophoretic deposition (EPD).

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Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 by Gérald Thouand, Robert Marks


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