Read e-book online Biochemistry & Genetics: PreTest Self-Assessment & Review PDF

By Golder Wilson

ISBN-10: 0071375783

ISBN-13: 9780071375788

ISBN-10: 0071389377

ISBN-13: 9780071389372

Offers clinical scholars 500 questions, solutions, and causes to arrange for the biochemistry and genetics sections of the USMLE Step 1. the recent version comprises many new questions within the two-step scientific structure to simulate the USMLE Step 1.

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Extra info for Biochemistry & Genetics: PreTest Self-Assessment & Review

Sample text

Murray, pp 412–434. Scriver, pp 3–45. Sack, pp 3–29. ) Before DNA replication can actually begin, unwinding protein must open segments along the DNA double helix. A defective unwinding protein slows the overall rate of DNA synthesis, but does not alter the size of replicated DNA fragments. Defects in DNA synthesis or transcription may produce a phenotype of accelerated aging, as in Cockayne’s syndrome [216400 (usually defective in a transcription factor)]. After unwinding, DNA-directed RNA polymerase (primase) catalyzes the synthesis of a complementary RNA primer of approximately 50 to 100 bases on each DNA strand.

C. d. e. Detects specific base pairs Detects DNA molecules Detects RNA molecules Detects proteins Determines chromosome structure 37. The hypothetical “stimulin” gene contains two exons that encode a protein of 100 amino acids. They are separated by an intron of 100 bp beginning after the codon for amino acid 10. Stimulin messenger RNA (mRNA) has 5′ and 3′ untranslated regions of 70 and 30 nucleotides, respectively. A complementary DNA (cDNA) made from mature stimulin RNA would have which of the following sizes?

The process can be simply reversed by a photoreactivating enzyme that cleaves the dimers and yields the original bases. Blue light is required for this. Alternatively, the dimer is removed. A UV-specific excinuclease nicks the dimer on its 5′ side. DNA polymerase I replicates the damaged sequence, while the damaged sequence swings out. Finally, the damaged piece is hydrolyzed by the 5′ to 3′ exonuclease activity of the DNA polymerase I. DNA ligase then joins the new piece to the original DNA at the cleavage site.

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Biochemistry & Genetics: PreTest Self-Assessment & Review by Golder Wilson


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